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    1. The hepatoprotective effects were determined by serum biochemistry profiles aspartate aminotransferase AST, alanine aminotransferase ALT, alkaline phosphatase ALP, and triglyceride TG, liver antioxidant levels ferric reducing ability plasma FRAP, superoxide dismutase SOD, malondialdehyde MDA, nitric oxide NO, and reduced glutathione assays GSH and histopathological examination with hematoxylin and eosin H E staining lasix 40 mg

    2. Immunofluorescence of dissociated gut cultures was carried out as reported previously 8 dosage of ivermectin for dogs 84Вµl of tracr and crRNA were mixed with 10x injection buffer 5Вµl and 0, 5Вµl of streptococcus pyogenes Cas9 protein Engen Cas9 NLS, 20ВµM, New England Biosciences and subsequently incubated for 15 minutes at 37 C

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